Chen Chia-Ling, MSc.
Topic:
Signaling pathways in activated hematopoietic progenitors.
www.researchgate.net/profile/Chia_Chen11
Chen Chia-Ling Publications
Low c-Kit Expression Level Induced by Stem Cell Factor Does Not Compromise Transplantation of Hematopoietic Stem Cells.
Chen C-L, Faltusova K, Molik M, Savvulidi F, Chang K-T, Necas E. Biol Blood Marrow Transplant 2016, 22:1167–1172.
Abstract:
The c-Kit expression level is decreased in regenerating bone marrow, and such bone marrow performs poorly when co-transplanted with normal bone marrow. We asked whether diminished numbers of c-Kit receptors on hematopoietic stem and progenitor cells (HSPCs) after their internalization induced by the binding of the cytokine stem cell factor (SCF) would jeopardize transplantability of HSPCs. We used a battery of functional assays to evaluate the capacity of HSPCs with markedly different c-Kit expression levels to be transplanted. Surprisingly, our experiments testing the homing of transplanted HSPCs to bone marrow of recipient mice and their short-term and long-term engraftment did not reveal any defects in HSPCs with severely reduced numbers of c-Kit receptor molecules. This unexpected result can be ascribed to the fact that HSPCs exposed to SCF replace the consumed c-Kit receptors rapidly. This article demonstrates that exposure of HSPCs to SCF and diminished number of c-Kit receptors in their cell membranes do not compromise the capacity of HSPCs to reconstitute damaged hematopoietic tissue.
Hematopoiesis Remains Permissive to Bone Marrow Transplantation After Expansion of Progenitors and Resumption of Blood Cell Production.
Báječný, M., Chen C.-L., Faltusová, K., Heizer T., Sziskszai K., Páral P., Šefc, L., and Nečas, E. (2021)., Front. Cell Dev. Biol., https://doi.org/10.3389/fcell.2021.660617
Altered erythro-myeloid progenitor cells are highly expanded in intensively regenerating hematopoiesis.
K. Faltusová, C-L. Chen, T. Heizer, M. Báječný, K. Szikszai, P. Páral, F. Savvulidi, N. Renešová and E.Necas (2020), Front. Cell Dev. Biol. doi: 10.3389/fcell.2020.00098
Extramedullary Hematopoiesis (EMH) in Laboratory Animals: Offering an Insight Into Stem Cell Research.
Chiu SC, Liu HH, Chen CL,Cell Transplant. 2015; doi: 10.3727/096368915X686850.
Multichannel lens-free CMOS sensors for real-time monitoring of cell growth.
Chang KT, Chang YJ, Chen CL, Wang YN.; Electrophoresis. 2015; doi: 10.1002/elps.201400272.
Chen Chia-Ling Posters
The CD71/Sca-1 diagram is a useful tool for investigation of hematopoietic stem and progenitor cells.
Chen CL. 20th Student Scientific Conference, First Faculty of Medicine, Charles University in Prague. 2019.
The early response cells to bone marrow injury.
Chen CL, Faltusova K, Paral P, Molik M, Heizer T, Savvulidi F, Necas E.; ISEH, Frankfurt, 2017.